The Resource A comprehensive, system-biology analysis of the response of soybean root hairs and stripped roots to heat stress, by Josef Batek

A comprehensive, system-biology analysis of the response of soybean root hairs and stripped roots to heat stress, by Josef Batek

Label
A comprehensive, system-biology analysis of the response of soybean root hairs and stripped roots to heat stress
Title
A comprehensive, system-biology analysis of the response of soybean root hairs and stripped roots to heat stress
Statement of responsibility
by Josef Batek
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
Heat stress is likely to be a key factor in the negative impact of climate change on crop production. Heat stress significantly influences the functions of roots, which provide support, water and nutrients to other plant organs. Likewise, roots play an important role in the establishment of symbiotic associations with different microorganisms. Despite the physiological relevance of roots, few studies have examined their response to heat stress. In this study, we performed a comprehensive systems-biology analysis of the response of soybean root hairs and stripped roots to heat stress. The analysis include a genome-wide transcriptomic, proteomic and metabolomic analyses on isolated root hairs, which are a single, epidermal cell type, and compared their response to whole roots. We identified 2,013 genes differentially regulated in root hairs in response to heat stress. Our gene regulatory module analysis identified ten key modules that controlled the majority of the transcriptional response to heat stress. We also conducted proteomic analysis on membrane fractions isolated from roots and root hairs. These experiments identified a variety of proteins whose expression changed within 3 hours of application of heat stress. Most of these proteins were predicted to play a significant role in thermo-tolerance, as well as in chromatin remodeling and post-transcriptional regulation. The metabolomic analysis identified that heat stress alters the metabolic profile of soybean root hairs. In the current study, we observe the accumulation of amino acids and carbohydrates which is a common defense mechanism that plants employ to cope with heat stress. The data presented represent an in-depth analysis of the heat stress response of a single cell type in soybean
Cataloging source
MUU
http://library.link/vocab/creatorName
Batek, Josef
Degree
M.S.
Dissertation note
Thesis
Dissertation year
2015.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorDate
1951-
http://library.link/vocab/relatedWorkOrContributorName
Stacey, Gary
http://library.link/vocab/subjectName
  • Crop science
  • Plants
  • Heat shock proteins
  • Roots (Botany)
  • Soybean
Label
A comprehensive, system-biology analysis of the response of soybean root hairs and stripped roots to heat stress, by Josef Batek
Instantiates
Publication
Note
  • "A Thesis presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Master of Science."
  • Thesis supervisor: Dr. Gary Stacey
Bibliography note
Includes bibliographical references (pages 89-99)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
953100745
Extent
1 online resource (vi, 99 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (chiefly color)
Specific material designation
remote
System control number
(OCoLC)953100745
Label
A comprehensive, system-biology analysis of the response of soybean root hairs and stripped roots to heat stress, by Josef Batek
Publication
Note
  • "A Thesis presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Master of Science."
  • Thesis supervisor: Dr. Gary Stacey
Bibliography note
Includes bibliographical references (pages 89-99)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
953100745
Extent
1 online resource (vi, 99 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (chiefly color)
Specific material designation
remote
System control number
(OCoLC)953100745

Library Locations

  • Curtis Laws Wilson LibraryBorrow it
    400 West 14th Street, Rolla, MO, 65409, US
    37.955220 -91.772210
  • Missouri University of Science & Technology Library DepositoryBorrow it
    2908 Lemone Blvd, Columbia, MO, 65201, US
    38.919360 -92.291620
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