The Resource CAMV gene VI protein : a virulence factor and the host responses in Arabidopsis, by Weichang Yu

CAMV gene VI protein : a virulence factor and the host responses in Arabidopsis, by Weichang Yu

Label
CAMV gene VI protein : a virulence factor and the host responses in Arabidopsis
Title
CAMV gene VI protein
Title remainder
a virulence factor and the host responses in Arabidopsis
Statement of responsibility
by Weichang Yu
Creator
Subject
Language
eng
Summary
The gene VI protein (P6) of Cauliflower mosaic virus (CaMV) functions as a virulence factor in crucifers by eliciting a chlorosis symptom in infected plants. The ability to induce chlorosis has been associated previously with P6 through gene-swapping experiments between strains and through the analysis of transgenic plants that express P6. In this study, I characterized a variant of P6, derived from CaMV strain D4, which does not induce chlorosis upon transformation into Arabidopsis thaliana , and two other variants (W260 and CM1841) that induce strong chlorosis and stunting. The asymptomatic D4 P6 was proven to be fully functional. This work demonstrated that the virulence function of CaMV P6 was not related to its function as a Translational Transactivator (TAV), it also had implications for the CaMV gene expression strategies, and enabled us to analyze the functions of other CaMV genes not related to P6 in the disease expression. To characterize the host response to the CaMV P6, I profiled the host global gene expression in both the symptomatic W260 P6 and the asymptomatic D4 P6 transgenic Arabidopsis by a cDNA microarray. The microarray analysis revealed that the expression of 69 genes was affected by the more virulent W260 P6, whereas the expression of only 22 genes was affected by the D4 P6 in transgenic Arabidopsis. In general, genes involved in transcription, translation and stress response were induced, whereas genes involved in photosynthesis, hormone response, metabolism, and transport were suppressed in transgenic plants that expressed W260 P6. A cluster analysis coupled with a search of upstream regions of clustered genes, revealed two putative transcription factor binding motifs that may function in the induction of stress responses in transgenic plants that express P6. To visualize the subcellular location of CaMV P6, the GFP gene was fused to the C-terminus of P6 of the CaMV W260 isolate (G6GFP). The G6GFP fusion protein was able to form fluorescent inclusion bodies when expressed in agroinfiltrated N. edwardsonii leaves and in transgenic Arabidopsis. Furthermore, G6GFP appeared to have full TAV activity in an agroinfiltration assay. However, the G6GFP did not elicit HR in agroinfiltrated N. edwardsonii or elicit virus-like symptoms in transgenic Arabidopsis, likely due to the instability of the G6GFP transcript. Preliminary experiments indicated that co-infiltration of the wild type W260 P6 construct with pG6GFP could stabilize the G6GFP transcript
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1966-
http://library.link/vocab/creatorName
Yu, Weichang
Degree
Ph. D.
Dissertation year
2002.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Arabidopsis
  • Mosaic diseases
  • Virus diseases of plants
  • Chlorosis (Plants)
  • Plant viruses
Target audience
specialized
Label
CAMV gene VI protein : a virulence factor and the host responses in Arabidopsis, by Weichang Yu
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
52722887
Dimensions
29 cm
Dimensions
unknown
Extent
viii, 164 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
Label
CAMV gene VI protein : a virulence factor and the host responses in Arabidopsis, by Weichang Yu
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
52722887
Dimensions
29 cm
Dimensions
unknown
Extent
viii, 164 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote

Library Locations

  • Curtis Laws Wilson LibraryBorrow it
    400 West 14th Street, Rolla, MO, 65409, US
    37.955220 -91.772210
  • Missouri University of Science & Technology Library DepositoryBorrow it
    2908 Lemone Blvd, Columbia, MO, 65201, US
    38.919360 -92.291620
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